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Literature summary extracted from

  • Bevers, L.E.; Bol, E.; Hagedoorn, P.L.; Hagen, W.R.
    WOR5, a novel tungsten-containing aldehyde oxidoreductase from Pyrococcus furiosus with a broad substrate specificity (2005), J. Bacteriol., 187, 7056-7061.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.B3 Iron contains one [4Fe-4S] cluster per subunit Pyrococcus furiosus
1.2.7.B3 Tungsten one tungstobispterin cofactor per subunit Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.B3 67000
-
 2 * 67000, SDS-PAGE Pyrococcus furiosus
1.2.7.B3 135000
-
 non-denaturing PAGE Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.B3 additional information Pyrococcus furiosus ferredoxin can be the physiological electron acceptor ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.B2 Pyrococcus furiosus
-
-
-
1.2.7.B3 Pyrococcus furiosus E7FHH1 and Q8U0V2 E7FHH1: alpha-subunit, Q8U0V2: electron-transfer beta-subunit
-
1.2.7.5 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.B3
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.B3 15.6
-
 pH 8.4, 60°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.B2 crotonaldehyde + H2O + oxidized methyl viologen specific activity is 2.5% compared to the activity with formaldehyde Pyrococcus furiosus crotonate + reduced methyl viologen + H+
-
 ?
1.2.7.B2 formaldehyde + H2O + oxidized methyl viologen
-
 Pyrococcus furiosus formate + reduced methyl viologen + H+
-
 ?
1.2.7.B2 hexanal + H2O + oxidized methyl viologen specific activity is 2% compared to the activity with formaldehyde Pyrococcus furiosus hexanoate + reduced methyl viologen + H+
-
 ?
1.2.7.B3 2-ethylhexanal + H2O + oxidized benzyl viologen Vmax/Km is 56% compared to the value for hexanal Pyrococcus furiosus 2-ethylhexanoate + reduced benzyl viologen + H+
-
 ?
1.2.7.B3 2-methylbutyraldehyde + H2O + oxidized benzyl viologen Vmax/Km is 20% compared to the value for hexanal Pyrococcus furiosus 2-methylbutyrate + reduced benzyl viologen + H+
-
 ?
1.2.7.B3 2-methylvaleraldehyde + H2O + oxidized benzyl viologen Vmax/Km is 54% compared to the value for hexanal Pyrococcus furiosus 2-methylvalerate + reduced benzyl viologen + H+
-
 ?
1.2.7.B3 2-naphthaldehyde + H2O + oxidized benzyl viologen Vmax/Km is 6.7% compared to the value for hexanal Pyrococcus furiosus 2-naphthoate + reduced benzyl viologen + H+
-
 ?
1.2.7.B3 3-phenylbutyraldehyde + H2O + oxidized benzyl viologen Vmax/Km is 11% compared to the value for hexanal Pyrococcus furiosus 3-phenylbutyrate + reduced benzyl viologen + H+
-
 ?
1.2.7.B3 cinnamaldehyde + H2O + oxidized benzyl viologen Vmax/Km is 5.3% compared to the value for hexanal Pyrococcus furiosus cinnamate + reduced benzyl viologen + H+
-
 ?
1.2.7.B3 hexanal + H2O + 2 oxidized ferredoxin
-
 Pyrococcus furiosus hexanoate + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.B3 hexanal + H2O + oxidized methyl viologen
-
 Pyrococcus furiosus hexanoate + reduced methyl viologen + H+
-
 ?
1.2.7.B3 hydratropaldehyde + H2O + oxidized benzyl viologen Vmax/Km is 89% compared to the value for hexanal Pyrococcus furiosus ? + reduced benzyl viologen + H+
-
 ?
1.2.7.B3 isobutyraldehyde + H2O + oxidized benzyl viologen Vmax/Km is 17% compared to the value for hexanal Pyrococcus furiosus isobutyrate + reduced benzyl viologen + H+
-
 ?
1.2.7.B3 additional information ferredoxin can be the physiological electron acceptor Pyrococcus furiosus ?
-
 ?
1.2.7.B3 additional information it is distinguished from aldehyde ferredoxin oxidoreductase (EC 1.2.7.5) by its low activity and affinity for crotonaldehyde, wich is one of the best substrates of aldehyde ferredoxin oxidoreductase. It is distinguished from formaldehyde ferredoxin oxidoreductase by its low activity and affinity for formaldehyde, which is one of the best substrates of formaldehyde ferredoxin oxidoreductase. It shows high affinity for several substituted and nonsubstituted aliphatic and aromatic aldehydes with various chain lengths. Very low activity (Vmax/Km is less than 5% compared to activity with hexanal): formaldehyde, crotonaldehyde, acetaldehyde, glutaraldehyde, 2-methoxybenzaldehyde. No activity with glyceraldehyde 3-phosphate Pyrococcus furiosus ?
-
 ?
1.2.7.5 crotonaldehyde + H2O + oxidized methyl viologen
-
 Pyrococcus furiosus crotonate + H+ + reduced methyl viologen
-
 ?
1.2.7.5 formaldehyde + H2O + oxidized methyl viologen specific activity is 17% compared to the activity with crotonaldehyde Pyrococcus furiosus formate + H+ + reduced methyl viologen
-
 ?
1.2.7.5 hexanal + H2O + oxidized methyl viologen specific activity is 5.7% compared to the activity with crotonaldehyde Pyrococcus furiosus hexanoate + H+ + reduced methyl viologen
-
 ?
1.2.7.5 additional information the enzyme is most active on aldehydes derived from amino acids Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.B3 homodimer 2 * 67000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.2.7.B2 FOR
-
 Pyrococcus furiosus
1.2.7.B3 WOR5
-
 Pyrococcus furiosus
1.2.7.5 AOR
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.B3 80
-
-
 Pyrococcus furiosus

General Information

EC Number General Information Comment Organism
1.2.7.B2 physiological function the enzyme plays a role in peptide fermentation Pyrococcus furiosus
1.2.7.5 physiological function the enzyme plays a role in peptide fermentation Pyrococcus furiosus